HPLC MS Method for Analysis of Benzalkonium Chloride on Primesep SB  Column

HPLC Method for Analysis of Benzalkonium chloride on Primesep SB by SIELC Technologies

 HPLC MS Method for Analysis of Benzalkonium Chloride on Primesep SB  Column
HPLC Method for Analysis of Benzalkonium chloride on Primesep B Column by SIELC Technologies


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Benzalkonium chloride

Benzalkonium chloride is a quaternary ammonium compound and belongs to the class of chemicals known as cationic surfactants. It is often used as a disinfectant, antiseptic, and preservative in various products, including pharmaceuticals, personal care products, and surface disinfectants.

Function: It has antimicrobial properties and is effective against bacteria, viruses, and fungi. It disrupts the cell membranes of microorganisms, leading to their inactivation.

Common Uses:

  • Disinfection of surfaces in healthcare settings.
  • Preservative in ophthalmic solutions and nasal sprays.
  • Ingredient in some antiseptic wipes and hand sanitizers.
  • Preservation agent in various personal care products.

Variants: There are different variants of Benzalkonium chloride with varying alkyl chain lengths, and the specific composition may affect its properties and applications.

Benzalkonium can be retained, and analyzed using a Primesep SB mixed-mode stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium formate as a buffer. Detection is achieved using UV and LC MS positive mode

ColumnPrimesep SB, 2.1 x 100 mm, 5 µm, 100 A
Mobile PhaseGradient MeCN – 30 – 70%, 10 min
BufferAmmonium formate pH 3.0 – 40 mM
Flow Rate0.2ml/min
DetectionUV 262 nm, SIM + 276, 304, 332, 360
Samples0.0025 mg/mL in MeCN/H2O – 50/50%
Injection volume1 µl
LOD*1 ppb
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Alkaloid, Quaternary ammonium
Analyzing CompoundsBenzalkonium chloride

Application Column

Primesep SB

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Benzalkonium chloride

Application Detection:
UV Detection
LC MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.