HPLC Method for Analysis of Benzalkonium chloride on Primesep SB by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Benzalkonium chloride
Benzalkonium chloride is a quaternary ammonium compound and belongs to the class of chemicals known as cationic surfactants. It is often used as a disinfectant, antiseptic, and preservative in various products, including pharmaceuticals, personal care products, and surface disinfectants.
Function: It has antimicrobial properties and is effective against bacteria, viruses, and fungi. It disrupts the cell membranes of microorganisms, leading to their inactivation.
Common Uses:
- Disinfection of surfaces in healthcare settings.
- Preservative in ophthalmic solutions and nasal sprays.
- Ingredient in some antiseptic wipes and hand sanitizers.
- Preservation agent in various personal care products.
Variants: There are different variants of Benzalkonium chloride with varying alkyl chain lengths, and the specific composition may affect its properties and applications.
Benzalkonium can be retained, and analyzed using a Primesep SB mixed-mode stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium formate as a buffer. Detection is achieved using UV and LC MS positive mode
| Column | Primesep SB, 2.1 x 100 mm, 5 µm, 100 A |
| Mobile Phase | Gradient MeCN – 30 – 70%, 10 min |
| Buffer | Ammonium formate pH 3.0 – 40 mM |
| Flow Rate | 0.2ml/min |
| Detection | UV 262 nm, SIM + 276, 304, 332, 360 |
| Samples | 0.0025 mg/mL in MeCN/H2O – 50/50% |
| Injection volume | 1 µl |
| LOD* | 1 ppb |
| Class of Compounds | Alkaloid, Quaternary ammonium |
| Analyzing Compounds | Benzalkonium chloride |
Application Column
Primesep SB
Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
LC MS Detection
