HPLC Method for Analysis of Phthalic acid on Primesep B Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Phthalic Acid on Primesep B by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

HPLC Method for Analysis of Phthalic Acid on Primesep B Column


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Phthalic Acid

Phthalic acid is an aromatic dicarboxylic acid with the chemical formula C₆H₄(CO₂H)₂. It consists of a benzene ring with two carboxylic acid groups (-COOH) attached to adjacent carbon atoms (in the ortho position). It is mainly produced by the oxidation of naphthalene or o-xylene.

Uses
Phthalic acid is primarily used as a precursor to other chemicals, including:

Phthalic anhydride: Used in the production of plasticizers for PVC (polyvinyl chloride).
Alkyd resins: Important in the manufacture of paints and coatings.
Dyes and pigments: Phthalic acid derivatives are often used in the production of dyes.

Phthalic Acid can be retained, separated and analyzed using a Primesep B mixed-mode stationary phase column. The analysis employs a gradient method with a simple mobile phase comprising water, acetonitrile (MeCN), and phosphoric acid as a buffer. This method allows for detection using UV 200 nm.

You can find detailed UV spectra of Phthalic Acid and information about its various lambda maxima by visiting the following link.

ColumnPrimesep B, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 30/70%
BufferH2SO4 – 0.1%
Flow Rate1.0 ml/min
DetectionUV 200 nm
Limit of Detection5 ppb
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Acid
Analyzing CompoundsPhthalic Acid

Application Column

Primesep B

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Phthalic Acid
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.