HPLC Method for Analysis of Glucose 6-phosphate and Fructose 6-phosphate on Newcrom B Column by SIELC Technologies.
Separation type: Liquid Chromatography Mixed-mode
Glucose 6-phosphate, also known as Robison ester, and Fructose 6-phosphate, known as Neuberg ester, are derivatives of glucose and fructose (respectively) that form during cellular metabolism. Glucose 6-phosphate is first formed by cells to ensure it does not diffuse out of the cell, and then through the process of glycolysis, it’s isomerized into Fructose 6-phosphate.
These 2 phosphorylated sugars can be retained, separated, and analyzed on a mixed-mode Newcrom B column with a mobile phase consisting of water, Acetonitrile (MeCN), and Formic acid (FA). This analytical method can be analyzed via any evaporative detector, including ELSD, CAD, and ESI-MS with high resolution and peak symmetry.
High Performance Liquid Chromatography (HPLC) Method for Analyses of Glucose 6-phosphate and Fructose 6-phosphate
|Column||Newcrom B, 4.6×150 mm, 5 µm, 100A|
|Mobile Phase||MeCN – 50%|
|Buffer||Formic Acid – 0.5%|
|Flow Rate||1.0 ml/min|
|Peak Retention Time||11.4, 12.2 min|
|Class of Compounds||Sugar|
|Analyzing Compounds||Glucose 6-phosphate and Fructose 6-phosphate|
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.Select options