HPLC Method for Analysis of Glucose 6-phosphate and Fructose 6-phosphate on Newcrom B Column by SIELC Technologies.
Separation type: Liquid Chromatography Mixed-mode
Glucose 6-phosphate, also known as Robison ester, and Fructose 6-phosphate, known as Neuberg ester, are derivatives of glucose and fructose (respectively) that form during cellular metabolism. Glucose 6-phosphate is first formed by cells to ensure it does not diffuse out of the cell, and then through the process of glycolysis, it’s isomerized into Fructose 6-phosphate.
These 2 phosphorylated sugars can be retained, separated, and analyzed on a mixed-mode Newcrom B column with a mobile phase consisting of water, Acetonitrile (MeCN), and Formic acid (FA). This analytical method can be analyzed via any evaporative detector, including ELSD, CAD, and ESI-MS with high resolution and peak symmetry.
High Performance Liquid Chromatography (HPLC) Method for Analyses of Glucose 6-phosphate and Fructose 6-phosphate
Condition
| Column | Newcrom B, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN – 50% |
| Buffer | Formic Acid – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD, 50C |
| Peak Retention Time | 11.4, 12.2 min |
Description
| Class of Compounds | Sugar |
| Analyzing Compounds | Glucose 6-phosphate and Fructose 6-phosphate |
Application Column
Newcrom B
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsGlucose 6-Phosphate
