HPLC Method for Analysis of N6-(Δ2-Isopentenyl)adenine on Primesep 100 Column

High Performance Liquid Chromatography (HPLC) MS Method for Analysis of N6-(Δ2-Isopentenyl)adenine on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

HPLC Method for Analysis of N6-(Δ2-Isopentenyl)adenine on Primesep 100 Column


 High Performance Liquid Chromatography (HPLC) Method for Analysis of N6-(Δ2-Isopentenyl)adenine

N6-(Δ²-Isopentenyl)adenine (iP) is a cytokinin, a plant hormone that regulates cell division and growth. Like trans-Zeatin, it is part of the adenine-derived cytokinin family and plays a significant role in promoting plant development and differentiation.

This compound is particularly important in both natural plant physiology and agricultural biotechnology, where it is applied to stimulate growth or improve crop yields.

N6-(Δ2-Isopentenyl)adenine can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 210 nm.

You can find detailed UV spectra of N6-(Δ2-Isopentenyl)adenine and information about its various lambda maxima by visiting the following link.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN – 50%
BufferH2SO4 -0.2%
Flow Rate1.0 ml/min
DetectionUV 205 nm
Sample0.1 mg/ml
DiluentMeCN/H2O- 50/50%
LOD*3 ppb
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Phytohormone
Analyzing CompoundsN6-(Δ2-Isopentenyl)adenine

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
N6-(Δ2-Isopentenyl)adenine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.