HPLC Method for the Analysis of Tirzepatide in Pharmaceutical Formulation Mounjaro on Primesep B Column

HPLC Method for Tirzepatide on Primesep B by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Tirzepatide

Tirzepatide is a synthetic peptide that functions as a dual agonist of the GLP-1 and GIP receptors. It is used in the treatment of type 2 diabetes and obesity. By mimicking the actions of both glucagon-like peptide-1 (GLP-1) and gastric inhibitory polypeptide (GIP), tirzepatide enhances insulin secretion, reduces blood sugar levels, promotes satiety, and aids in weight loss.

Tirzepatide can be retained and analyzed using the Primesep B stationary phase column. The analysis utilizes an gradientc method with a simple mobile phase consisting of water, acetonitrile (MeCN), and TFA. Detection is performed using UV at 280 nm.

ColumnPrimesep B, 3.2 x 50 mm, 5 µm, 100 A
Mobile PhaseGradient MeCN – 30-70%, 10 mn
BufferTFA – 0.1%
Flow Rate0.5 ml/min
DetectionUV 280 nm
LOD24 ppb

*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of CompoundsGLP-1 Receptor Agonist
Analyzing CompoundsTirzepatide

Application Column

Primesep B

Column Diameter: 3.2 mm
Column Length: 50 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Tirzepatide
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.