HPLC Method for Analysis of Proguanil on Primesep 100 Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Proguanil hydrochloride on Primesep 100 by SIELC Technologies

HPLC Method for Analysis of Proguanil on Primesep 100 Column
HPLC Method for Analysis of Proguanil hydrochloride on Primesep 100 Column by SIELC Technologies

Proguanil is an antimalarial medication with the chemical formula C11H17Cl2N5. It is often used in combination with other drugs, such as atovaquone. It is used for both prevention and treatment of malaria. Proguanil is a prodrug, and its active form inhibits the development of the malaria parasite in the liver. You can find detailed UV spectra of Proguanil and information about its various lambda maxima by visiting the following link.

These biguanides exhibit various pharmacological activities, and while some are primarily used for antidiabetic purposes, others have applications in antiviral or antimalarial treatments. It’s important to note that the safety and efficacy of these drugs depend on proper medical supervision and adherence to prescribed dosages.

Proguanil hydrochloride can be retained and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and ammonium formate as a buffer. This method allows for detection using UV at 240 nm

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O 75/25%
BufferAmFm pH 3.0  – 30 mM
Flow Rate1.0 ml/min
DetectionUV 240 nm
Samples 0.5 mg/ml
Injection volume2 µl
LOD*8 ppb
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
Class of Compounds
Biguanides
Analyzing CompoundsProguanil hydrochloride

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Proguanil hydrochloride
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.