HPLC – MS Method for Analysis of Terbinafine on Primesep B Column

HPLC Method for Analysis of Terbinafine on Primesep B by SIELC Technologies

HPLC - MS Method for Analysis of Terbinafine on Primesep B Column  by SIELC Technologies

 High Performance Liquid Chromatography (HPLC) Method for Analysis of  Terbinafine

Terbinafine is an antifungal medication commonly used to treat fungal infections, particularly those caused by dermatophytes. Here are some key points about terbinafine:

Mechanism of Action: Terbinafine is classified as an allylamine antifungal. It works by inhibiting the enzyme squalene epoxidase, which is important in the fungal ergosterol synthesis pathway. This disruption results in a deficiency of ergosterol in the fungal cell membrane and an accumulation of squalene, which is toxic to the fungus.

Terbinafine can be retained, and analyzed on a Primesep B mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a ammonium format as a buffer. This analysis method can be detected using UV at 283 nm, an Evaporative Light Scattering Detector (ELSD), or any other evaporative detection method (CAD, ESI-MS)

ColumnPrimesep B, 2.1 x 100 mm, 5 µm, 100 A
Mobile PhaseGradient MeCN – 20-50%, 8 min
BufferAmmonium Formate pH 3.0-20 mM
Flow Rate0.2 ml/min
DetectionUV, 282 nm, SIM 292+
Class of Compounds
Analyzing CompoundsTerbinafine

Application Column

Primesep B

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.