HPLC Method for Analysis of Terbinafine on Primesep B Column

HPLC Method for Analysis of Terbinafine on Primesep B by SIELC Technologies

HPLC Method for Analysis of Terbinafine on Primesep B Column by SIELC Technologies

 High Performance Liquid Chromatography (HPLC) Method for Analysis of  Terbinafine

Terbinafine is an antifungal medication primarily used to treat fungal infections of the skin and nails. It belongs to the allylamine class of antifungals.

Terbinafine is classified as an allylamine antifungal agent.

The allylamines are a class of antifungal drugs that inhibit the enzyme squalene epoxidase, which is an essential component of fungal cell membrane synthesis. By inhibiting this enzyme, terbinafine prevents the fungi from synthesizing ergosterol, a vital component of their cell membranes. This leads to an accumulation of squalene and results in fungal cell death.

Terbinafine is commonly prescribed for the treatment of fungal infections of the skin, hair, and nails, such as tinea pedis (athlete’s foot), tinea cruris (jock itch), tinea corporis (ringworm), and onychomycosis (fungal infection of the nails).

Here are some key aspects and indications for terbinafine:

  1. Mechanism of Action: Terbinafine works by inhibiting squalene epoxidase, an enzyme crucial to the biosynthesis of ergosterol in fungi. Ergosterol is an essential component of the fungal cell membrane. By interfering with its production, terbinafine disrupts the cell membrane’s integrity, leading to fungal cell death.

Terbinafin can be retained, and analyzed on a Primesep B mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a ammonium format a buffer. This analysis method can be detected in the UV 270 nm.

ColumnPrimesep B, 2.1 x 100 mm, 5 µm, 100 A
Mobile PhaseGradient MeCN – 20-50%, 10 min
BufferAmmonium Formate pH 4.0-20 mM
Flow Rate0.2 ml/min
DetectionUV, 282 nm
Sample concentration0.5 mg/ml
Injection volume1 µl
LOD 8 ppb
Class of Compounds
Analyzing CompoundsTerbinafine

Application Column

Primesep B

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:

Application Detection:
UV Detection
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