Separation type: Liquid Chromatography Mixed-mode
Salicylic acid (SA) is an o-hydroxybenzoic acid, synthesized by both plants and microorganisms. SA acts as a critical plant hormone regulating various processes, including growth and development, flowering, thermogenesis, ion uptake, stomatal movement, photosynthesis, and plant immunity. SA also has medical applications as a topical treatment for a wide variety of skin conditions, including warts, dandruff, and acne. Using a Newcrom BH mixed-mode column and a mobile phase consisting of water, Acetonitrile (MeCN), and a Phosphoric acid (H3PO4) buffer, Salicylic acid can be retained, measured, and analyzed. This analysis method can be UV detected at 200 nm with high resolution with high resolution and peak symmetry.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Salicylyc Acid
| Column | Newcrom BH, 4.6×150 mm, 100A |
| Mobile Phase | MeCN – 40% |
| Buffer | H3PO4 – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200nm |
| Class of Compounds | Acid, Hydrophilic |
| Analyzing Compounds | Salicylic Acid |
Application Column
Newcrom BH
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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