Sunset Yellow FCF, also known as Orange Yellow S, C.I. 15985, or Yellow 6, is a petroleum-derived orange azo dye. When in powder form, it is Reddish-orange, but when dissolved in water, the liquid becomes bright yellow. The dye is banned or restricted in Norway, Finland and Sweden. Obelisc N is a column with very polar characteristics. It contains embedded acidic and basic ionizable groups and can retain Sunset Yellow. The method is LC/MS and UV compatible and can be used as a general approach for analyzing similar compounds.
| Column | Obelisc N, 3.2×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | AmFm pH 3.0 – 70 mM |
| Flow Rate | 0.6 ml/min |
| Detection | UV, 525, 630 nm |
| Class of Compounds |
Dye, Acid, Hydrophilic, Ionizable, Zwitterionic |
| Analyzing Compounds | Allura Red, Brilliant Blue |
Application Column
Obelisc N
SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
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