Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies
HPLC Method for Analysis of Polyglutamic Acid on BIST A by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Polyglutamic Acid
Polyglutamic acid (PGA) is a biopolymer consisting of repeating units of Glutamic acid. PGA can be found and used in a wide variety of industries due to its biodegradable, non-toxic properties, including in the food, medical, and wastewater industries. Using SIELC’s newly introduced BIST™ method, PGA can be retained easily on a negatively-charged, cation-exchange BIST™ A column. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as N,N’-Dimethylpiperazine (DMP), which acts as a bridge, linking the negatively-charged anion analytes to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Other positively-charged buffers that can generate BIST™ include TMDAP, Calcium acetate, and Magnesium acetate. Using this new and unique analysis method, PGA can be retained with high selectivity and great peak shape. This method can be detected and is compatible with ELSD, CAD, and Mass Spectrometry (LC-MS).
Condition
| Column | BIST™ A, 4.6×50 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN |
| Buffer | N,N’-Dimethylpiperazine formate pH 4.0 |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD, 70C |
Description
| Class of Compounds | Acid, Polymer, Peptide |
| Analyzing Compounds | Polyglutamic Acid |
Application Column
BIST A
BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.
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