HPLC Method for Analysis of Citric Acid and Potassium Bromide on Newcrom BH Column

Separation type: Liquid Chromatography Mixed-mode
 

High Performance Liquid Chromatography (HPLC) Method for Analysis of Citric Acid and Potassium Bromide

 

Citric Acid is a naturally ocurring organic acid found in citrus fruits; it is also an intermediate in the citric acid cycle of aerobic organisms. It is used industrially as an acidity regulator, flavoring, detergent, and more than 2 million tons are produced annually. Potassium Bromide used to be a popular anticonvulsant and sedative, and is now mainly used as an antiepileptic medication for dogs. Citric acid and Potassium Bromide can be retained, separated, and analyzed on a mixed-mode Newcrom BH column with a mobile phase consisting of water, Acetonitrile (MeCN), and Trifluoroacetic acid (TFA). This analytical method can be UV detected at 210 nm with high resolution and peak symmetry. This method is also compatible with Mass Spectrometry (MS), ELSD, and CAD. The Citric acid was detected via negative Electrospray Ionization (ESI-) at an m/z of 191.

Condition
Column Newcrom BH, 3.2×100 mm, 3 µm, 100A
Mobile Phase MeCN – 10%
Buffer TFA – 0.1%
Flow Rate 0.5 ml/min
Detection UV 210 nm, ESI

 

Description
Class of Compounds
 Acid
Analyzing Compounds Citric Acid, Potassium Bromide

Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Bromide
Citric Acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.