HPLC Method for Analysis of Choline on Obelisc R Column

Separation type: Liquid Chromatography Ion Exclusion

High Performance Liquid Chromatography (HPLC) Method of Choline

Choline is a water-soluble vitamin-like essential nutrient.  It is a constituent of lecithin, which is present in many plants and animal organs.  Choline is a nutrient related to the B-vitamin family.  First discovered in 1862, it wasn’t classified as an essential nutrient until very recently.  It can be retained on an Obelisc R column, which has both positive and negative ion-pairs embedded in the stationary phase, allowing for the fine tuning and separation of a wide range of compounds with different ionic properties.  Choline can be determined isocratically using a simple MS-compatible mobile phase of acetonitrile (ACN) and water with Ammonium Formate (AmFm) buffer and detected by ELSD, CAD or LC/MS.

ColumnObelisc R, 4.6×150 mm, 5 µm, 100A
Mobile PhaseMeCN/H2O – 20/80%
BufferAmFm pH 4.0- 5 mM
Flow Rate1.0 ml/min
DetectionCAD (Corona) MS- compatible mobile phase
Class of Compounds
Drug,  Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing CompoundsCholine

Application Column

Obelisc R

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:


Application Detection:
CAD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.