HPLC Method for Analysis of Adipic Acid on Newcrom B Column

Separation type: Liquid Chromatography Mixed-mode

 

 

HPLC Method for Analysis of Adipic Acid on Newcrom B

High Performance Liquid Chromatography (HPLC) Method for Analysis of Adipic Acid

Adipic acid (or Hexanedioic acid) is a heavily used, industrial dicarboxylic acid used as a pre-cursor for the mass-production of nylon. It is also used as an ingredient in timed-release drug capsules and as a tart flavoring agent in foods. Adipic acid can be retained and analyzed on a mixed-mode Newcrom B column with a mobile phase consisting of water, Acetonitrile (MeCN), and Sulfuric acid (H2SO4). This analytical method can be UV detected at 200 nm with high resolution and peak symmetry.

Condition
Column Newcrom B, 4.6×150 mm,  100A
Mobile Phase MeCN/H2O -5/95%
Buffer H2SO4 – 0.02%
Flow Rate 1.0 ml/min
Detection UV 200 nm

Description
Class of Compounds Acid, Hydrophilic
Analyzing Compounds Adipic Acid

 

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Adipic Acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.