HPLC Method for Analysis of 4-ethoxyaniline on Primesep 100 Column

Separation type: Liquid Chromatography Mixed-mode

 


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High Performance Liquid Chromatography (HPLC) Method for Analysis of 4-ethoxyaniline

4-Ethoxyaniline, also known as p-Phenetidine, is one of three Phenetidine isomers, and is commonly used as an intermediate product in drug and dye synthesis. 4-Ethoxyaniline can be retained and analyzed on a mixed-mode Primesep 100 column with a mobile phase consisting of water, Acetonitrile (MeCN), and Sulfuric acid (H2SO4). This analytical method can be UV detected at 200 nm with high resolution and peak symmetry.

Condition
Column Promesep 100, 4.6×150 mm,  100A
Mobile Phase MeCN – 40%
Buffer H2SO4 – 0.2%
Flow Rate 1.0 ml/min
Detection UV 200nm

Description
Class of Compounds Aromatic amine
Analyzing Compounds 4-ethoxyaniline

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
4-Ethoxyaniline
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.