HPLC Determination of Uridine Diphospate Glucose on Newcrom BH

HPLC Method for Uridine-5'-diphosphate-glucose on Newcrom BH by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Fructose 1,6 Bisphosphate

Uridine Diphosphate Glucose (UDP-glucose) is an an important nucleotide sugar with the chemical formula C15H24N2O17P2. It is involved in metabolic processes in the cell. It is a precursor to glycogen, UDP-galactose, and UDP-glucuronic acid, as well as other polysacchrides and glycosphingolipids.

UDP-Galactose can be detected in the low UV regime. Using a Newcrom BH mixed-mode column and a mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid (H2SO4) buffer, UDP-galactose can be retained, measured, and analyzed. This analysis method can be UV detected at 210 nm.

Condition
Column Newcrom BH, 2.1 x 150 mm, 3 µm, 100 A, dual ended
Mobile Phase MeCN – 10%
Buffer H2SO4 – 0.2%
Flow Rate 1.0 ml/min
Detection UV 210 nm

 

Description
Class of Compounds Sugar, Phospate
Analyzing Compounds  Uridine-5'-diphosphate-glucose

 

 

Application Column

Newcrom BH

Column Diameter: 2.1 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Uridine-5′-diphosphate-glucose
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.