HPLC Determination of Uridine Diphospate Glucose on Newcrom BH

Separation type: Liquid Chromatography Mixed-mode

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High Performance Liquid Chromatography (HPLC) Method for Analysis of Fructose 1,6 Bisphosphate
Uridine Diphosphate Glucose (UDP-glucose) is an an important nucleotide sugar involved in metabolic processes in the cell. It is a precursor to glycogen, UDP-galactose, and UDP-glucuronic acid, as well as other polysacchrides and glycosphingolipids. UDP-Galactose can be detected in the low UV regime. Using a Newcrom BH mixed-mode column and a mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid (H2SO4) buffer, UDP-galactose can be retained, measured, and analyzed. This analysis method can be UV detected at 210 nm.

Condition

Column Newcron BH, 4.6x150 mm, 3 µm, 100A
Mobile Phase MeCN - 10%
Buffer H2SO4 - 0.2%
Flow Rate 1.0 ml/min
Detection UV 210 nm
 

Description

Class of Compounds Sugar, Phospate
Analyzing Compounds Uridine-5'-diphosphate-glucose
   
Application Analytes:
Uridine-5′-diphosphate-glucose