HPLC Determination of Tebuconazole on Newcrom B

HPLC Method for Tebuconazole on Newcrom B by SIELC Technologies

HPLC Determination of Tebuconazole on Newcrom B Column_1214

High Performance Liquid Chromatography (HPLC) Method for Analysis of Tebuconazole.

Tebuconazole is a triazole fungicide with the chemical formula C16H22ClN3O. It is used against to plant pathogenic fungi. Despite the U.S. Food and Drug Administration considering this fungicide safe, it is considered a possible carcinogen with the rating of C. According to the World Health Organization, it has moderate toxicity and is slightly hazardous.

Tebuconazole can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an ammonium formate buffer. Detection is performed using UV.

Condition
Column Newcrom B, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 40/60%
Buffer Gradient AmFm pH 3.0 –  20 mM
Flow Rate 1.0 ml/min
Detection UV, 275 nm

Description
Class of Compounds Hydrophobic, Neutral, Herbicide, Pesticide
Analyzing Compounds Tebuconazole

Application Column

Newcrom B

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Tebuconazole
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.