HPLC Determination of Tartrazine (Yellow 5) on Newcrom BH Column

Separation type: Liquid Chromatography Mixed-mode HPLC.cloud View on hplc.cloud Tartrazine is a yellow food coloring agent. It’s a trisodium salt, and like many multi-charged molecules tends to exhibit significant tailing using reverse-phase HPLC columns. By using a mixed-mode column, specifically designed in mind for multiple charges, Newcrom BH can retain tartrazine with perfect peak shape using a mobile phase of methanol (MeOH) and water with sulfuric acid (H2SO4) to facilitate ion-exchange. UV detection at 430nm. High Performance Liquid Chromatography (HPLC) Method for Analysis of Tartrazine


Column Newcrom BH, 4.6x50 mm, 5 µm, 100A
Mobile Phase MeOH/H2O - 75/25%
Buffer H2SO4 - 1%
Flow Rate 1.0 ml/min
Detection UV 430  nm


Class of Compounds Acid, Dyes
Analyzing Compounds Tartrazine Yellow 5
Application Analytes:
Tartrazine (Yellow 5)