HPLC Determination of Tartrazine (Yellow 5) on Newcrom BH Column

HPLC Method for Tartrazine (FD&C Yellow 5) on Newcrom BH by SIELC Technologies

 

High Performance Liquid Chromatography (HPLC) Method for Analysis of Tartrazine (FD&C Yellow 5).

Tartrazine is a yellow food coloring agent. It’s a trisodium salt, and like many multi-charged molecules tends to exhibit significant tailing using reverse-phase HPLC columns. By using a mixed-mode column, specifically designed in mind for multiple charges, Newcrom BH can retain tartrazine with perfect peak shape using a mobile phase of methanol (MeOH) and water with sulfuric acid (H2SO4) to facilitate ion-exchange. UV detection at 430nm.

You can find detailed UV spectra of Yellow 5 and information about its various lambda maxima by visiting the following link.

Condition
Column Newcrom BH, 4.6 x 50 mm, 5 µm, 100 A, dual ended
Mobile Phase MeOH/H2O – 75/25%
Buffer H2SO4 – 1%
Flow Rate 1.0 ml/min
Detection UV 430  nm

Description
Class of Compounds Acid, Dyes
Analyzing Compounds Tartrazine (FD&C Yellow 5)

 

Application Column

Newcrom BH

Column Diameter: 4.6 mm
Column Length: 50 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Tartrazine (FD&C Yellow 5)
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.