HPLC Determination of Tartrazine (Yellow 5) on Newcrom BH Column

Separation type: Liquid Chromatography Mixed-mode


Tartrazine is a yellow food coloring agent. It’s a trisodium salt, and like many multi-charged molecules tends to exhibit significant tailing using reverse-phase HPLC columns. By using a mixed-mode column, specifically designed in mind for multiple charges, Newcrom BH can retain tartrazine with perfect peak shape using a mobile phase of methanol (MeOH) and water with sulfuric acid (H2SO4) to facilitate ion-exchange. UV detection at 430nm.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Tartrazine


Column Newcrom BH, 4.6×50 mm, 5 µm, 100A
Mobile Phase MeOH/H2O – 75/25%
Buffer H2SO4 – 1%
Flow Rate 1.0 ml/min
Detection UV 430  nm


Class of Compounds Acid, Dyes
Analyzing Compounds Tartrazine Yellow 5


Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Tartrazine (FD&C Yellow 5)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.