HPLC Determination of Sodium Dodecyl Sulfate on Primesep B4 Column

HPLC Determination of Sodum Dodecyl Sulfate

Sodium dodecyl sulfate, or sodium lauryl sulfate, is a surfactant used in cleaning and hygienic products. It has a twelve carbon hydrophobic tail attached to a polar sulfate. Dodecyl sulfate can be retained on Primesep B4 column in under 5 minutes with an MS-compatible mobile phase. Primesep B4 is a short carbon chain reverse-phase (RP) mixed-mode column with embedded basic ion-pairing groups.



Column Primesep B4, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 60/40%
Buffer AmFm pH 3.0 – 30 mM
Flow Rate 1.0 ml/min
Detection CAD (Corona) MS- compatible mobile phase



Class of Compounds
Hydrophobic, Base, Ionizable, Anionic surfactant
Analyzing Compounds Sodium Dodecyl Sulfate


Application Column

Primesep B4

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Sodium dodecyl sulfate
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.