HPLC Determination of Rifampicin on Newcrom A column

HPLC Method for Rifampicin on Newcrom A by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Rifampicin.

Rifampicin is an antibiotic used alongside other antibiotics to treat several types of bacterial infections like tuberculosis and Legionnaires’ disease. It works by inhibiting the bacteria’s RNA production and is on the World Health Organisation’s (WHO) List of essential medicines. It’s chemical formula is  C43H58N4O12.

It can be retained in HPLC on Newcrom A mixed-mode column. The retention characteristics can be controlled by the organic modifier used in the mobile phase, with methanol (MeOH) having a longer retention time in comparison to acetonitrile (ACN). UV detection at 275nm.

Condition
Column Newcrom A, 4.6 x 50 mm, 3 µm, 100 A, dual ended
Mobile Phase MeOH, MeCN
Buffer H2SO4 – 0.5%
Flow Rate 1.0 ml/min
Detection UV 275 nm

 

Description
Class of Compounds Hydrophobic, Drugs, Antibiotics
Analyzing Compounds Rifampicin

Application Column

Newcrom A

Column Diameter: 4.6 mm
Column Length: 50 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Rifampicin
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.