HPLC Determination of Rifampicin on Newcrom A column

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Rifampicin is an antibiotic used alongside other antibiotics to treat several types of bacterial infections like tuberculosis and Legionnaires’ disease. It works by inhibiting the bacteria’s RNA production and is on the World Health Organisation’s (WHO) List of essential medicines. It can be retained in HPLC on Newcrom A mixed-mode column. The retention characteristics can be controlled by the organic modifier used in the mobile phase, with methanol (MeOH) having a longer retention time in comparison to acetonitrile (ACN). UV detection at 275nm.


Column Newcrom A, 4.6×50 mm, 3 µm, 100A
Mobile Phase MeOH, MeCN
Buffer H2SO4 – 0.5%
Flow Rate 1.0 ml/min
Detection UV 275 nm



Class of Compounds Hydrophobic, Drugs, Antibiotics
Analyzing Compounds Rifampicin

Application Column

Newcrom A

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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