Polyethyleneimine (PEI) is a polymer used in a wide range of applications. It is difficult to analyze in HPLC due to several factors. It’s a mixture of compounds with different lengths and therefore different number of charges. It also lacks a UV chromophore. New PEI column was designed specifically for the analysis of polyethyleneimine by ion-exclusion and size-exclusion mechanisms with copper complex to allow for UV detection. The method uses a mobile phase of acetonitrile (ACN) and water with ammonium formate buffer (AmFm) and UV detection at 285nm.
MEASURING PEI Polyethylenimine in biological samples
| Column | PEI, 4.6×250 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 40/60% |
| Buffer | AmFM pH 3.0 – 20 mM |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 285 nm |
| Class of Compounds |
Polymer, Basic, Hydrophilic, Ionizable |
| Analyzing Compounds | PEI |
Application Column
PEI
SIELC Technologies offers custom phases for customers who require specific separations not achievable with commercially available HPLC phases. Considering the vast array of compounds and mixtures requiring analysis, tailored LC phases can significantly enhance separation results for unique and challenging applications. To learn more about our special custom LC phases designed for your specific separation needs, please contact SIELC Technologies at research@sielc.com. Our team of experts is ready to guide you through the process and create a custom solution that addresses your particular chromatographic challenges.
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