HPLC Method for Separation of Glutathione on Primesep 100 by SIELC Technologies
Glutathione is a tripeptide composed of three amino acids: glutamate, cysteine, and glycine. It’s often referred to as the body’s “master antioxidant” because of its vital role in maintaining cellular health and preventing oxidative stress.
Here are some key functions and characteristics of glutathione:
- Antioxidant Function: Glutathione is the most prevalent endogenous antioxidant, protecting cells from damage by neutralizing harmful free radicals and reactive oxygen species. It also regenerates other antioxidants, like vitamins C and E, from their oxidized forms.
- Detoxification: Glutathione contributes to the neutralization of many different types of harmful substances, such as pollutants, heavy metals, and some drugs, thereby aiding in the detoxification process.
- Immune System Support: It plays a crucial role in the proper function of white blood cells, including T cell lymphocytes—the foundation of adaptive immunity.
- Health and Disease: Low levels of glutathione have been linked to several diseases, including cancer, neurodegenerative disorders such as Parkinson’s disease, cardiovascular diseases, and HIV/AIDS. Additionally, glutathione levels typically decrease with age, which can contribute to the aging process.
Glutathione is primarily synthesized in the liver and then distributed to other tissues in the body. It can also be obtained from some foods, especially fruits, vegetables, and meats.
Glutathione can be retained on Primesep 100 mixed-mode column using a mobile phase of Acetonitrile (ACN), water and Trifluoroacetic acid (TFA) buffer. This analysis method can be detected via Refraction Index (RI) Detection, mass spectrometry (MS), evaporative light scattering detection (ELSD) and Charged aerosol detection (CAD).
High Performance Liquid Chromatography (HPLC) Method for Analysis of Glutathione
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN/H2O – 5/95% |
| Buffer | TFA – 0.4% |
| Flow Rate | 1.0 ml/min |
| Detection | RI |
| Class of Compounds | Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid |
| Analyzing Compounds | Glutathione |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
