HPLC Determination of Carbaryl on Newcrom B Column

HPLC.cloud View on hplc.cloud Carbaryl, also known as Sevin, is an insecticide that works as a cholinesterase inhibitor. Carbaryl can be retained on Newcrom B mixed-mode column in HPLC analysis with an isocratic method by using a mobile phase of acetonitrile (ACN) and water with ammonium formate (AmFm) as buffer, making the analytical method MS-compatible. Can also be UV detected at 280nm.


Column Newcrom B, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 50/50%
Buffer AmFm pH 3.0 -  20 mM
Flow Rate 1.0 ml/min
Detection UV, 280 nm


Class of Compounds Hydrophobic, Herbicide, Pesticide
Analyzing Compounds Carbaryl

Application Analytes: