Carbaryl, also known as Sevin, is an insecticide that works as a cholinesterase inhibitor. Carbaryl can be retained on Newcrom B mixed-mode column in HPLC analysis with an isocratic method by using a mobile phase of acetonitrile (ACN) and water with ammonium formate (AmFm) as buffer, making the analytical method MS-compatible. Can also be UV detected at 280nm.
Column | Newcrom B, 4.6×150 mm, 5 µm, 100A |
Mobile Phase | MeCN/H2O – 50/50% |
Buffer | AmFm pH 3.0 – 20 mM |
Flow Rate | 1.0 ml/min |
Detection | UV, 280 nm |
Class of Compounds | Hydrophobic, Herbicide, Pesticide |
Analyzing Compounds | Carbaryl |
Application Column
Newcrom B
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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