HPLC Determination of Carbaryl on Newcrom B Column

View on hplc.cloud

Carbaryl, also known as Sevin, is an insecticide that works as a cholinesterase inhibitor. Carbaryl can be retained on Newcrom B mixed-mode column in HPLC analysis with an isocratic method by using a mobile phase of acetonitrile (ACN) and water with ammonium formate (AmFm) as buffer, making the analytical method MS-compatible. Can also be UV detected at 280nm.


Column Newcrom B, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 50/50%
Buffer AmFm pH 3.0 –  20 mM
Flow Rate 1.0 ml/min
Detection UV, 280 nm


Class of Compounds Hydrophobic, Herbicide, Pesticide
Analyzing Compounds Carbaryl

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.