HPLC Determination of Acid red 1 on Newcrom BH Column

HPLC.cloud View on hplc.cloud Acid Red 1, also known as Red 2G, is a synthetic dye that’s used as a food additive. It has been banned in several countries due to safety concerns because it forms aniline in the body that can interfere with the function of hemoglobin. Acid Red 1 can be retained on HPLC mixed-mode Newcrom BH column using a mobile phase consisting of methanol (MeOH) and water with sulfuric acid (H2SO) buffer. Vis detection at 535nm.


Column Newcom BH, 4.6x50 mm, 5 µm, 100A
Mobile Phase MeOH/H2O - 75/25%
Buffer H2SO4 - 0.5%
Flow Rate 1.0 ml/min
Detection Vis 535 nm


Class of Compounds Acid, Dyes
Analyzing Compounds Acid red 1
Application Analytes:
C.I. Acid Red 1