HPLC Method for C.I. Acid Red 1 on Newcrom BH by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of C.I. Acid Red 1.
Acid Red 1, also known as Red 2G, is a synthetic dye that’s used as a food additive. It has been banned in several countries due to safety concerns because it forms aniline in the body that can interfere with the function of hemoglobin. Acid Red 1 can be retained on HPLC mixed-mode Newcrom BH column using a mobile phase consisting of methanol (MeOH) and water with sulfuric acid (H2SO) buffer. Vis detection at 535nm.
| Column | Newcrom BH, 4.6 x 50 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeOH/H2O – 75/25% |
| Buffer | H2SO4 – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | Vis 535 nm |
| Class of Compounds | Acid, Dyes |
| Analyzing Compounds | C.I. Acid Red 1 |
Application Column
Newcrom BH
Column Diameter: 4.6 mm
Column Length: 50 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
C.I. Acid Red 1Application Detection:
Vis Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
