Acid Red 1, also known as Red 2G, is a synthetic dye that’s used as a food additive. It has been banned in several countries due to safety concerns because it forms aniline in the body that can interfere with the function of hemoglobin. Acid Red 1 can be retained on HPLC mixed-mode Newcrom BH column using a mobile phase consisting of methanol (MeOH) and water with sulfuric acid (H2SO) buffer. Vis detection at 535nm.
| Column | Newcom BH, 4.6×50 mm, 5 µm, 100A |
| Mobile Phase | MeOH/H2O – 75/25% |
| Buffer | H2SO4 – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | Vis 535 nm |
| Class of Compounds | Acid, Dyes |
| Analyzing Compounds | Acid red 1 |
Application Column
Newcrom BH
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsApplication Analytes:
C.I. Acid Red 1
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