HPLC Determination of 6-methyl-5-hepten-2-one on Newcrom R1 Column

HPLC Determination of 6-methyl-5-hepten-2-one Sulcatone is a ketone used as a mosquito attractant. It is a volatile oil component found in citrus. It can be retained on a Newcrom R1reverse-phase column with a mobile phase of acetonitrile (ACN) and water with sulfuric acid (H2SO4) buffer. The analytical method is MS-compatible and can also use UV detection at 200nm.


Column Newcrom R1, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 50/50%
Buffer H2SO4 - 0.1 %
Flow Rate 1.0 ml/min
Detection UV, 200 nm


Class of Compounds Hydrophobic, Mosquito attractant
Analyzing Compounds 6-methyl-5-hepten-2-one
Application Analytes: