HPLC Determination of Polyhexanide (PHMB) on PEI Column

  Polyhexanide (polyhexamethylene biguanide, PHMB) is a polymer used as a disinfectant and antiseptic. We estimated that limit of detection is about 2 ppm for this compounds with this method. The peak of polyhexanide fractionated on several peaks due to difference in length of the polymer components.


Column PEI , 4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 40/60%
Buffer AmFm pH 3.0- 20 mM
Flow Rate 0.5 ml/min
Detection UV, 236 nm


Class of Compounds Polymer, Hydrophilic, Ionizable, Antiseptic, Disinfectant
Analyzing Compounds Polyhexanide (PHMB)
Application Analytes:
Polyhexanide PHMB