HPLC Determination of Polyhexanide (PHMB) on PEI Column


 

Polyhexanide (polyhexamethylene biguanide, PHMB) is a polymer used as a disinfectant and antiseptic. We estimated that limit of detection is about 2 ppm for this compounds with this method.

The peak of polyhexanide fractionated on several peaks due to difference in length of the polymer components.

 

Condition
Column PEI , 4.6×250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 40/60%
Buffer AmFm pH 3.0- 20 mM
Flow Rate 0.5 ml/min
Detection UV, 236 nm

 

Description
Class of Compounds
 Polymer, Hydrophilic, Ionizable, Antiseptic, Disinfectant
Analyzing Compounds Polyhexanide (PHMB)

 

Application Column

PEI

SIELC Technologies offers custom phases for customers who require specific separations not achievable with commercially available HPLC phases. Considering the vast array of compounds and mixtures requiring analysis, tailored LC phases can significantly enhance separation results for unique and challenging applications. To learn more about our special custom LC phases designed for your specific separation needs, please contact SIELC Technologies at research@sielc.com. Our team of experts is ready to guide you through the process and create a custom solution that addresses your particular chromatographic challenges.

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Application Analytes:
Polyhexanide PHMB
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.