HPLC Method for Polyhexanide PHMB on PEI by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Polyhexanide PHMB
Polyhexanide (polyhexamethylene biguanide, PHMB) is a polymer used as a disinfectant and antiseptic. We estimated that limit of detection is about 2 ppm for this compounds with this method.
The peak of polyhexanide fractionated on several peaks due to difference in length of the polymer components.
| Column | PEI, 4.6 x 250 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 40/60% |
| Buffer | AmFm pH 3.0- 20 mM |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 236 nm |
| Class of Compounds |
Polymer, Hydrophilic, Ionizable, Antiseptic, Disinfectant |
| Analyzing Compounds | Polyhexanide PHMB |
Application Column
PEI
Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
Polyhexanide PHMBApplication Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
