Omeprazole is a proton pump inhibitor, a medication that decreases the amount of acid produced in the stomach. Omeprazole has a bactericidal effect on Helicobacter pylori.
Obelisc R column which has both positive and negative ion-pairs embedded in the stationary phase allows for fine tuning and separation of a wide range of compounds with different ionic properties. Omeprazole was separated isocratically using a simple MS-compatible mobile phase of acetonitrile (ACN) and water with Ammonium Acetate (AmAc) buffer. Can also be UV detected at 300nm.
| Column | Obelisc R, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN -35% |
| Buffer | Ammonium Acetate pH 5.5 – 10 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 300 nm |
| Column | Obelisc R, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN -20-50% in 15 min + 5 min hold |
| Buffer | Ammonium Acetate pH 5.5 – 10 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 300 nm |
| Class of Compounds |
Drug |
| Analyzing Compounds | Omeprazole |
Application Column
Obelisc R
SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
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