| CAS Number | 119-61-9 |
|---|---|
| Molecular Formula | C13H10O |
| Molecular Weight | 182.223 |
| InChI Key | RWCCWEUUXYIKHB-UHFFFAOYSA-N |
| LogP | 3.18 |
| Synonyms |
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Applications:
Separation of Benzophenone on Newcrom R1 HPLC column
February 16, 2018
Benzophenone can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsUSP Methods for the Analysis of Ibuprofen using a Legacy L1 Column
June 21, 2012
Application Notes: Ibuprofen is the most commonly used analgesic. It is a vasoconstrictor and fever reducer. According to the USP methods, ibuprofen contains no less than 97% and no more than 103% ibuprofen based on a dried basis. The USP HPLC method for the separation of procainamide was developed on Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing Ci18 ligand. Support for the material is spherical silica gel with particles size 3-10 um and pore size of 100-120A. Resolution between critical pairs corresponds to rules and specifications of USP.
Application Columns: Legacy L1 C18 HPLC column
Application compounds: Ibuprofen and benzophenone
Mobile phase: .01M H3PO4/MeCN (55/45)
Detection technique: UV
Reference: USP35: NF30
| Column | Legacy L1, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeOH/H2O – 45/55% |
| Buffer | H3PO4 |
| Flow Rate | 1.5 ml/min |
| Detection | UV, 230 nm |
| Class of Compounds |
Drug, Nonsteroidal anti-inflammatory drug, Hydrophobic, Ionizable, Neutral |
| Analyzing Compounds | Ibuprofen, benzophenone |
Application Column
Legacy L1
SIELC's family of Legacy columns is based on the United States Pharmacopeia's (USP) published chromatographic methods and procedures. Numerous brands have columns used in USP reference standards and methods. USP has created various designations to group together columns with similar types of packing and properties in the solid phase. SIELC's Legacy columns adhere to these strict requirements and properties, allowing you to easily replace older columns that are no longer available without needing to significantly modify your method or SOPs.
Select optionsIbuprofen
