 |
Step 6 |
We have established that your compound is a hydrophobic strong base with one basic group. Below is an example of a separation of a similar compound. This example can be used a starting condition in your method development.
Hydrophobic basic compounds can be analyzed by any Primesep column. Cation-exchange columns (Primesep 100, 200, P, C, A) provide very strong retention due to both RP and IE combined retention’s. Short columns with a strong mobile phase, which contain high organic and high buffer concentrations, are typically required when analyzing hydrophobic basic compounds. Anion exchange columns (Primesep D, B2) can also be used. They will decrease retention significantly because of the repulsion phenomena of the positively charged column surface and the positive charge of the analyte itself. Smaller buffer concentration and smaller organic concentration in the mobile phase is required in this type of separation. Good peak symmetry and high efficiency is typical even for very difficult basic compounds.
|
 |
|
