weak base

Step 5

We have established that your compound is a hydrophilic weak base. Below is an example of a separation of a similar compound. This example can be used a starting condition in your method development.

Hydrophilic basic compounds can be analyzed by any Primesep column. Anion-exchange columns (Primesep 100, 200, P, C, A) provide good peak shape and efficiency due to the charge repulsion phenomena since both the analyte and column surfaces have the same negative charge. Both the analyte and surface can be neutral depending on the pH of the mobile phase. Anion exchange columns (Primesep D, B2) can also be used. They will increase retention and would usually require a higher buffer concentration, especially at a pH over 4 where weak acidic analytes are ionized.