HPLC Analysis of Zearalenone and Ochratoxin A on Primesep D Mixed-Mode Column

Zearalenone and Ochratoxin A are harmful mycotoxins commonly found in grains and other foods. They were separated using the Primesep B2 reversed-phase anion-exchange mode column. Retention can be controlled by adjusting concentration of both acetonitrile and buffer.


Column Primesep B2, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer Formic Acid
Flow Rate 1.0 ml/min
Detection UV, 250 nm


Class of Compounds Base, Acid, Hydrophilic, Ionizable
Analyzing Compounds Zearalenone, Ochratoxin A
Application Analytes:
Ochratoxin A