HPLC Analysis of Epinephrine and Epinephrine Sulfonate on Primesep AB Column

 

Epinephrine and epinephrine sulfonate were analyzed using Primesep AB, a reverse-phase column with both acidic and basic embedded ion-pairing groups. This method is LC/MS compatible.

 

Condition

Column Primesep AB, 4.6×250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 5/95%
Buffer H3PO4 – 0.25%
Flow Rate 1.0 ml/min
Detection UV, 280 nm

 

Description

Class of Compounds
Drug, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Epinephrine, Epinephrine Sulfonate

 

Application Column

Primesep AB

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Epinephrine
Epinephrine Sulfonate

Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.