USP Methods for the Analysis of Pyridoxine for the Legacy L1 Column

  Application Notes: Pyridoxine is part of the vitamin B complex group. Pyridoxine is important in the body’s daily function as it regulates many enzymatic reactions. The USP HPLC method for the separation of pyridoxine was developed on Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing C18 ligand. Support for the material is spherical silica gel with particles size 3-10 um and pore size of 100-120A. Resolution between critical pairs corresponds to rules and specifications of UPS.   Application Columns: Legacy L1 C18 HPLC column Application compounds: Pyridoxine Mobile phase: AcOH/MeOH/H2O/Hexanesulfonate (2/47/153/1.2) Detection technique: UV Reference: USP35- NF30


Column Legacy L1, 4.6x250 mm, 5 µm, 100A
Mobile Phase AcOH/MeOH/H2O/Hexanesulfonate 2/47/153/1.2
Buffer Hexanesulfonate
Flow Rate 1.0 ml/min
Detection UV, 280 nm


Class of Compounds Drug, Vitamin B₆, Hydrophobic, Ionizable
Analyzing Compounds Pyridoxine
Application Analytes:

Vitamin B6 (Pyridoxine)