USP Methods for the Analysis of Praziquantel using the Legacy L1 Column

Praziquantel is an anthelmintic drug effective against flatworms. The drug is used to treat diseases in humans, mammals, and fish. Praziquantel and related impurities can be analyzed by reversed-phase chromatography according to the USP, United States Pharmacopeia,   manuscript. It requires a reversed-phase HPLC column, classified as L1. This method was developed on the Legacy L1 column and can be used for analysis of various molecules according to USP.


Column Legacy L1, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 60/40%
Buffer No
Flow Rate 1.0 ml/min
Detection UV, 210 nm



Class of Compounds
Drug, Anthelmintic, Hydrophobic
Analyzing Compounds Praziquantel


Application Column

SIELC Legacy L1 HPLC column

Legacy L1

SIELC's family of Legacy columns is based on the United States Pharmacopeia's (USP) published chromatographic methods and procedures. Numerous brands have columns used in USP reference standards and methods. USP has created various designations to group together columns with similar types of packing and properties in the solid phase. SIELC's Legacy columns adhere to these strict requirements and properties, allowing you to easily replace older columns that are no longer available without needing to significantly modify your method or SOPs.

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SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to so we can ensure you get optimal results for your compound/s of interest.