USP Methods for the Analysis of Epinephrine Using the Legacy L1 Column

  Application Notes: Epinephrine is a synthetic adrenaline used to treat cardiac arrest and anaphylaxis. Phenylephrine is a decongestant and is often used instead of pseudoephedrine. According to the USP methods epinephrine contains no less than 97% and no more than 100.5 percent of epinephrine calculated on a dried basis. The USP HPLC method for the separation of phenylephrine and epinephrine was developed on Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing C18 ligand. Support for the material is spherical silica gel with particles size 3-10 um and pore size of 100-120A. Resolution between critical pairs corresponds to rules and specifications of USP. Application Columns: Legacy L1 C18 HPLC column Application compounds: Epinephrine and Phenylphrine Mobile phase: Water/MeOH (50/50) with 1% 1-Octanesulfonic acid adjust to pH 3.0 with H3PO4 Detection technique: UV Reference: USP35: NF30


Column Legacy L1, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeOH/MeOH - 50/50%
Buffer 1-Octanesulfonic acid adjust to pH 3.0 with H3PO4 - 0.1%
Flow Rate 1.0 ml/min
Detection UV, 270 nm


Class of Compounds Drug, Antiarrhythmic, Hydrophobic, Ionizable
Analyzing Compounds Epinephrine
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Application Analytes:

Application Detection:
UV Detection