Simultaneous Determination of Sodium and Sulfate Ions on Obelisc N Mixed-mode HILIC Column

Inorganic cations and anions are usually analyzed by two different methods – cation-exchange and anion-exchange. This requires development and validation of two methods. Both ions can be analyzed on mixed-mode HILIC column with ELSD detection. Both ions are retained by combination of HILIC and ion-exchange mechanism.


Column Obelisc N, 4.6×50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmFm, pH 3.0
Flow Rate 1.0 ml/min
Detection ELSD



Class of Compounds
Acid, Hydrophilic, Ionizable
Analyzing Compounds Sodium, Sulfate Ions


Application Column

Obelisc N

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to so we can ensure you get optimal results for your compound/s of interest.