Separation of Glyphosate by Cation Exchange and Anion Exchange

Primesep 100 separates a mixture of glyphosate and isopropylamine by HPLC using cation exchange as a retention mechanism. The peak order of this mixture is reversed on a Primesep B column which uses anion exchange. This combination offers an alternative to ion chromatography for the simultaneous analysis of complex herbicide mixtures. Glyphosate, other phosphonomethyl herbicides, and their amine salts can be analyzed with a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) and evaporative light scattering detection (ELSD).


Column Primesep B, Primesep 100, 4.6x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer TFA
Flow Rate 0.5 ml/min
Detection ELSD


Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Glyphosate, Isppropylamine
Application Analytes: