HPLC Separation of Acetylcholines

Primesep C separates acetylcholine and methylacetylcholine from their chloride counter ion with baseline resolution by a combination of cation exchange, complex formation, and hydrophobic interactions. The mass spec (LC/MS) compatible method uses a mobile phase of water, acetonitrile (MeCN, ACN) and ammonium acetate with evaporative light scattering detection (ELSD).

 

Condition
Column Primesep C, 3.2×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAc ph 4.1
Flow Rate0.5  ml/min
Detection ELSD

 

Description
Class of Compounds
 Drug
Analyzing Compounds Acetylcholine, Methylacetylcholine

 

Application Column

Primesep C

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Acetylcholine
Methylacetylcholine
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.