Mixed-Mode Retention and Separation of Ammonia, Primary and Secondary Amines on Primesep A Column

Separation type: Liquid Chromatography Mixed-mode



Ammonia, primary and secondary amines are common compounds used as building blocks in organic synthesis. Derivatives of these compounds are used in agriculture, pharmaceutical, and chemical manufacturing. All compounds in this application are basic and hydrophilic in nature. Problems in analysis include poor peak shape, lack of retention on C18 columns, and absence of chromophores in ammonia, primary and secondary amines. Analysis of hydrophilic amines requires derivatization to provide hydrophobicity/retention and UV activity, ion-pairing reagents to provide retention. Presence of residual silanols will affect peak shape. Ammonia, methylamine, morpholine, ethylamine, diethylamine, and butylamine can be analyzed on a Primesep A HPLC column with trifluoroacetic acid in the mobile phase. Compounds in the mixture can be monitored by ELSD.

Application Column

Primesep A

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Ammonia
Butylamine
Diethylamine
Ethylamine
Methylamine
Morpholine
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.