HPLC Separation of Uracil and Uridine in HILIC Mode on Primesep S2 Column

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Uracil and uridine are separated based on their polarity. The mechanism of retention is pure HILIC mode. Retention time is controlled by varying the amount of ACN. Buffer concentration and pH will affect ionization and polarity of the stationary phase, and thus indirectly affect retention of neutral compounds. Column is compatible with all general detection techniques.

Condition
Column Primesep S2, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 80/20%
Buffer AmAc
Flow Rate 1.0 ml/min
Detection ELSD, 50C UV 250 nm

 

Description
Class of Compounds
 Nucleosides,  Hydrophilic, Ionizable
Analyzing Compounds Uracil, Uridine

 

Application Column

Primesep S2

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Uracil
Uridine
Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.