HPLC Separation of Uracil and Uridine in HILIC Mode on Primesep S2 Column

chr_310.gif Uracil and uridine are separated based on their polarity. The mechanism of retention is pure HILIC mode. Retention time is controlled by varying the amount of ACN. Buffer concentration and pH will affect ionization and polarity of the stationary phase, and thus indirectly affect retention of neutral compounds. Column is compatible with all general detection techniques.


Column Primesep S2, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 80/20%
Buffer AmAc
Flow Rate 1.0 ml/min
Detection ELSD, 50C UV 250 nm


Class of Compounds Nucleosides,  Hydrophilic, Ionizable
Analyzing Compounds Uracil, Uridine
Application Analytes: