HPLC Separation of Terbufos on Obelisc Mixed-Mode Columns

Terbufos is an extremely toxic organophosphate insecticide and nematacide. Obelisc R was used to retain terbufos and separate it from impurities. Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning separations. Method is LC/MS compatible and can be used as a general approach for analyzing terbufos, other organophosphate insecticides, and dozens of other pesticides.


Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 4,5- 20-50 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 250 nm


Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Terbufos
Application Analytes: