HPLC Separation of Isomers of Aminobutyric Acids

Primesep C separates the isomers of aminobutyric acids by a combination of reversed-phase and ionic interaction mechanisms. alpha-Aminobutyric acid, beta-Aminobutryic acid, and gamma-Aminobutyric acid (GABA) are baseline resolved without ion-pair reagents. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) ammonium acetate with evaporative light scattering detection (ELSD).


Column Primesep C,  3.2x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAc pH 4.1
Flow Rate 0.5 ml/min
Detection ELSD


Class of Compounds Acid
Analyzing Compounds Alpha-Aminobutyric acid, Beta-Aminobutryic acid, Gamma-Aminobutyric acid (GABA)

Application Analytes:
Aminobutyric Acids
alpha-Aminobutyric Acid
beta-Aminobutyric Acid
gamma-Aminobutyric Acid (GABA)