Epinephrine and epinephrine sulfonate are separated on combination of Primesep 100 and Primesep SB HPLC columns. Primesep 100 column retains epinephrine by combination of reversed-phase and cation-exchange mechanisms. Epinephrine sulfonate is retained by weak reversed-phase mechanism. Due to strong zwitter-ionic nature of epinephrine sulfonate, ionic interaction is not available for this molecule. Compounds can be monitored by UV, ELSD, CAD or LC/MS.
| Column | Primesep SB, Primesep 100 4.6×250 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 70/30% |
| Buffer | AmFm |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds |
Drug, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Epinephrine, Epinephrine Sulfonate |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select options
Primesep SB
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsEpinephrine Sulfonate
UV Detection
