HPLC Separation of Aromatic Compounds (PAH) on Mixed-Mode and Reverse Phase Columns

Aromatic hydrocarbons are hydrophobic compounds which are well retained on any reverse column. Retention time is adjusted by the amount of ACN. Change in buffer concentration or buffer pH does not affect retention time. Method on Obelisc mixed-mode column shows retention and separation of PAHs by reverse phase mechanism. Change of pH is changing conformation and ionization of stationary phase on Obelisc R column, making it more or less hydrophobic. This changes interaction on the column and selectivity of separation. This method can be used for analysis of hydrophobic compounds and isomers by reverse phase mechanism, when fine tuning is required to achieve desired degree of separation.

Application Column

Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

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Application Analytes:

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.