HPLC Separation of Aromatic Boronic Acids

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Boronic acid is alkyl, or aryl substituted, boric acid. Boronic acid can form complexes with sugars, amines, and amino acids. Four aromatic boronic acids were separated on a Primesep P mixed-mode reversed-phase cation-exchange column. Primesep P column also has an aromatic fragment which provides pi-pi interaction. Compounds can be monitored by UV, ELSD, CAD and LC/MS. Various mobile phases can be employed for HPLC analysis of boronic acids.

 

 

Condition

Column Primesep P, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer H2SO4
Flow Rate 1.0 ml/min
Detection UV 270 nm

 

Description

Class of Compounds
Drug, Acid, Hydrophobic, Ionizable
Analyzing Compounds Chlorohenyl-4-boronic acid, Naphtalene-1-boronic acid, Biphenyl-4-boronic acid, 4-(1-naphthyl)phenylboronic acid

 

SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Primesep P

The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

Select options
Application Analytes:
4-(1-naphthyl)phenylboronic acid
4-Chlorophenylboronic acid
Biphenyl-4-boronic acid
Naphthalene-1-boronic acid

Application Detection:
UV Detection