HPLC Separation of Aromatic Boronic Acids

chr_298.gif Boronic acid is alkyl, or aryl substituted, boric acid. Boronic acid can form complexes with sugars, amines, and amino acids. Four aromatic boronic acids were separated on a Primesep P mixed-mode reversed-phase cation-exchange column. Primesep P column also has an aromatic fragment which provides pi-pi interaction. Compounds can be monitored by UV, ELSD, CAD and LC/MS. Various mobile phases can be employed for HPLC analysis of boronic acids.    


Column Primesep P, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer H2SO4
Flow Rate 1.0 ml/min
Detection UV 270 nm


Class of Compounds Drug, Acid, Hydrophobic, Ionizable
Analyzing Compounds Chlorohenyl-4-boronic acid, Naphtalene-1-boronic acid, Biphenyl-4-boronic acid, 4-(1-naphthyl)phenylboronic acid
Application Analytes:
4-(1-naphthyl)phenylboronic acid
4-Chlorophenylboronic acid
Biphenyl-4-boronic acid
Naphthalene-1-boronic acid