HPLC Separation of 5-Methyltetrahydrofolic Acid and Degradation Products

5-MTHF is a form of folic acid, one of the B vitamins. Folic acid is an essential vitamin used in synthesis of genetic molecules (DNA, RNA) and for the metabolism of amino acids. The separation of 5-MTHF can be achieved using reverse-phase (RP) Primesep SB column by hydrophobic, anion-exchange and ion-exclusion mechanisms. The method can be used for quality control (QC), research and development (R&D), etc. Fine tuning can be achieved with variations in mobile phase, pH and buffer selection. UV detection at 290mn. 

Application Column

Primesep SB

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
5-Methyltetrahydrofolic Acid (5-MTHF)
Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.