HPLC Diazinon Analysis on Primesep 100 column

 

 

Diazinon is a thiophosphoric acid ester which was used to control cockroaches, fleas, and silverfish in residential buildings. Diazinon is considered to be toxic for vertebrates causing a number of observed symptoms such as convulsions, headaches, and vomiting. Diazinon was was outlawed for residential use in the U.S. in 2004, but can be used in agricultural industries. There is a lot of interest in analyzing diazinon: it was analyzed in an analysis of acidic compounds by the EURL (European Union Reference Library) using QuEChERS methodology. Primesep 100 and Obelisc R were used to separate diazinon with unique selectivity. Method is LC/MS compatible and can be replicated for dozens of pesticides.

 

 

Condition 

Column Primesep 100, 2.1×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 10-70%, 15 min
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min
Flow Rate 0.4 ml/min
Detection UV, 270 nm

 

Description

Class of Compounds
 Insecticide, Hydrophobic, Ionizable
Analyzing Compounds Diazinon

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Diazinon

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.