Succinic acid is a dicarboxylic acid that plays important role in citric acid cycle. Succinic acid is byproduct of sugar fermentation, and is present in wines and beer. In one of the forms it exists as a salt with sodium. Both sodium and succinic acid can be retained and separated on Primesep 100 column. Sodium is retained by cation-exchange mechanism, while succinic acid is separated by reverse phase mechanism. Method can be used for HPLC analysis of metal ions (sodium, potassium, lithium, calcium, etc) and carboxylic acids (hydrophobic carboxylic mono and diacids) in one chromatographic run. ELSD is detection of choice for non-UV active non-volatile compounds.
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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