Metformin is a biguanide-based anti-diabetic drug. It is a very polar organic compound that has no retention on reversed-phase column unless an ion-pairing reagent is employed. Metformin was retained on a Primesep 200 column based on cation-exchange mechanism. Method can be used for analysis of metformin and related substances in pharmaceutical formulations. Due to low UV activity a more UV-transparent mobile phase is required. Metformin can be also monitored by ELSD and LC/MS with corresponding mobile phase (TFA, ammonium formate or ammonium acetate).
| Column | Primesep 200, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | TFA |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 230 nm |
| Class of Compounds |
Drug, Anti-diabetic medication, Hydrophilic, Ionizable |
| Analyzing Compounds | Metformin |
Application Column
Primesep 200
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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