HPLC Analysis of Histones

Histones are highly alkaline proteins. A mixture of histones was separated on Promix MP HPLC column. Basic nature of stationary phase guarantees perfect shape of histones due to shielding effect of stationary phase. No silanol interaction was observed. Method can be used for analysis and isolation of histones.


Column Promix MP, 4.6×250 mm, 5 µm, 100A
Mobile Phase Gradient MeCN 10 – 40%, 40 min , 5 min hold
Buffer H2SO4 – 0.2%
Flow Rate 1.0 ml/min
Detection UV, 230 nm



Class of Compounds
Proteins, Hydrophobic, Ionizable
Analyzing Compounds Histones


Application Column

Promix MP

The Promix family of mixed-mode columns presents an innovative chromatography technology for the efficient resolution of peptides and proteins. This technology hinges on a unique blend of hydrophobic and ionic interactions, facilitated by a novel separation medium: a ligand bonded to a silica support, chemically combined with hydrophobic and ionic functional groups. This phase provides unparalleled selectivity and peak capacity. By independently adjusting the quantities of buffer and organic modifier, a virtually infinite number of separation conditions can be achieved, rendering it suitable for a wide array of biomolecules.

Select options
Application Analytes:

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.