HPLC Analysis of Histones

Histones are highly alkaline proteins. A mixture of histones was separated on Promix MP HPLC column. Basic nature of stationary phase guarantees perfect shape of histones due to shielding effect of stationary phase. No silanol interaction was observed. Method can be used for analysis and isolation of histones.


Column Promix MP, 4.6x250 mm, 5 µm, 100A
Mobile Phase Gradient MeCN 10 - 40%, 40 min , 5 min hold
Buffer H2SO4 - 0.2%
Flow Rate 1.0 ml/min
Detection UV, 230 nm


Class of Compounds Proteins, Hydrophobic, Ionizable
Analyzing Compounds Histones
Application Analytes: